Product Features：
1. Medium Pressure, High-Stability Pumping
- High-precision constant-flow piston pump with low pulsation and stable baseline
- Flow rate 0–25 mL/min, upgradeable to 50 mL/min for preparative purification
- Performance superior to peristaltic and metering pump systems
2. Full Wavelength UV Detection
- Full wavelength detector (190–850 nm) for flexible method development
- Grating monochromator design with wavelength accuracy < 0.2 nm
- Multiple flow cell path lengths available to match different purification needs
3. High-Quality Gradient and Sample Handling
- Multi-port valve design for buffer selection and precise sample injection
- Smooth, reproducible gradient formation, outperforming gradient cup systems
- Accurate injection without air introduction into the fluid path
4. Automated and Accurate Fraction Collection
- Fully automated fraction collector with high positional accuracy
- X-Y matrix structure without bottom motor for improved safety and stability
- Multiple collection modes: peak-based, volume-based and manual
5. Intelligent Software and Flexible Control
- Dedicated workstation with method database and one-click operation
- Supports panel, tablet, laptop and desktop control
- User management, sample management and system self-diagnostics included
6. Compact, Biocompatible System Design
- Compact footprint, suitable for installation inside chromatography cold rooms
- Biocompatible fluid path materials with good pressure resistance
- Supports unattended operation after pump or manual injection
Instrument Components:
1. Main Unit (including constant flow plunger pump, gradient valve, autosampler valve, sample selection valve, and collection valve);
2. UV Detector (including full-wavelength UV detector, flow cell, and digital-to-analog converter);
3. Conductivity Detector (including flow cell and digital-to-analog converter);
4. Small fully automated fraction collector (including main unit, test tube rack, and three-way solenoid valve);
5. ClearFirst-2000M protein purification workstation;
Optional Components:
1. pH Detector (optional, includes flow cell and digital-to-analog converter);
2. High-Pressure Upgrade Component, system pressure can be upgraded to 15MPa;
3. Chromatography Refrigeration Unit
Product Parameters:
| Solvent Compatibility | Commonly used aqueous buffer solutions for biomolecule purification |
| Flow Rate Range | 0-25 mL/min |
| Pressure Range | 0-5 MPa (50 bar, 725 psi) |
| Viscosity | Up to 5 cP |
| Wavelength Range | 190-850 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | ±0.2 nm |
| Optical Path | 2 mm |
| Conductivity Range | 0-999.9 mS/cm |
| Automatic Sample Injection Valve | Three-position seven-way valve, software controlled, supports quantitative loop injection, and supports sample injection, loading, and waste liquid functions. |
| Friction Collector | Small fully automated fraction collector. |
| Tract Rack | 18 mm tract rack (standard), 48 positions, suitable for 18*150 mm tract tubes and 15 mL cell centrifuge tubes. |
Product Applications:
1. Biomolecules: Separation and purification of proteins, nucleic acids, etc. (gel chromatography, ion exchange, hydrophobic interaction, affinity chromatography, etc.);
2. Separation and purification of high molecular weight impurities.
This instrument can perform various commonly used purification techniques, such as affinity chromatography, ion exchange chromatography, hydrophobic chromatography, and gel filtration. Therefore, this system can support the purification of various proteins and nucleic acids, including recombinant proteins, antibodies, vaccines, or natural proteins.
