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AY-CF-500M Protein Purification System is a compact, integrated low-pressure protein chromatography system developed for small-scale protein purification in life science laboratories.
Designed to replace GE AKTA Start and Bio-Rad BioLogic LP, ClearFirst-500M integrates pumping, UV detection, conductivity monitoring, automated fraction collection and intelligent control into a single platform.

AY-CF-500M Protein Purification System

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Product Detail

Product Features:

1. Stable and Reliable Flow Control
  • High-precision peristaltic pump with low pulsation and stable baseline
  • Flow rate 0–40 mL/min, suitable for both analytical and preparative purification


2. High-Quality Gradient Formation
  • Multi-valve buffer selection and sample loading
  • Smooth, reproducible gradients with performance superior to traditional gradient mixers


3. Dedicated Protein Detection
  • LED UV detector at 280 nm, optimized for protein absorbance
  • Long service life, high stability, minimal maintenance
  • High-precision conductivity detector for real-time monitoring of salt gradients


4. Automated Fraction Collection

  • Fully automatic fraction collector with high positional accuracy
  • X-Y matrix design without bottom motor for improved safety and stability
  • Multiple tube formats supported, flexible collection modes (peak, volume, manual)


5. Intelligent Control and Easy Operation

  • Android touchscreen operation for standalone use
  • Optional PC software for fully automated control
  • Built-in method library for one-click operation
  • Supports user management, system self-check and data management


6. Compact and Biocompatible Design

  • Small footprint, suitable for installation inside chromatography cold rooms
  • Biocompatible fluid path materials with good pressure resistance
  • Designed for long-term, routine laboratory use


Instrument Components:

1. Main Unit (including peristaltic pump, sample loading valve, gradient valve, and collection valve);

2. UV Detector (including LED single-wavelength UV detector, flow cell, and digital-to-analog converter);

3. Conductivity Detector (including flow cell and digital-to-analog converter);

4. ClearFirst (Android version) protein purification workstation;

5. Fully automated fraction collector (including main unit, test tube rack, and solenoid valve);


Optional Components:

1. pH Detector (optional, including flow cell and digital-to-analog converter);

2. Chromatography refrigerator;

3. ClearFirst (PC version) protein purification workstation;

4. Computer kit;


Product Parameters:

Solvent CompatibilityCommonly used aqueous buffer solutions for biomolecule purification
Flow Rate Range0-40 mL/min
Pressure Range0-0.2 MPa (2 bar, 30 psi)
Wavelength Range280 nm
Wavelength Accuracy±3 nm
Optical Path2 mm
Conductivity Range0-500 mS/cm
Friction CollectorSmall, fully automated fraction collector
Test Tube Racks
18 mm test tube rack (standard), 48 slots, suitable for 18*150 mm test tubes and 15 mL cell centrifuge tubes
1.5 mL test tube rack (optional), 130 slots, suitable for 1.5 mL centrifuge tubes
13 mm test tube rack (optional), 88 slots, suitable for 13*100 mm test tubes
15 mm test tube rack (optional), 80 slots, suitable for 15*150 mm test tubes and 5 mL cell centrifuge tubes
50 mL test tube rack (optional), 20 slots, suitable for 50 mL cell centrifuge tubes


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Product Detail

Product Features:

1. Stable and Reliable Flow Control
  • High-precision peristaltic pump with low pulsation and stable baseline
  • Flow rate 0–40 mL/min, suitable for both analytical and preparative purification


2. High-Quality Gradient Formation
  • Multi-valve buffer selection and sample loading
  • Smooth, reproducible gradients with performance superior to traditional gradient mixers


3. Dedicated Protein Detection
  • LED UV detector at 280 nm, optimized for protein absorbance
  • Long service life, high stability, minimal maintenance
  • High-precision conductivity detector for real-time monitoring of salt gradients


4. Automated Fraction Collection

  • Fully automatic fraction collector with high positional accuracy
  • X-Y matrix design without bottom motor for improved safety and stability
  • Multiple tube formats supported, flexible collection modes (peak, volume, manual)


5. Intelligent Control and Easy Operation

  • Android touchscreen operation for standalone use
  • Optional PC software for fully automated control
  • Built-in method library for one-click operation
  • Supports user management, system self-check and data management


6. Compact and Biocompatible Design

  • Small footprint, suitable for installation inside chromatography cold rooms
  • Biocompatible fluid path materials with good pressure resistance
  • Designed for long-term, routine laboratory use


Instrument Components:

1. Main Unit (including peristaltic pump, sample loading valve, gradient valve, and collection valve);

2. UV Detector (including LED single-wavelength UV detector, flow cell, and digital-to-analog converter);

3. Conductivity Detector (including flow cell and digital-to-analog converter);

4. ClearFirst (Android version) protein purification workstation;

5. Fully automated fraction collector (including main unit, test tube rack, and solenoid valve);


Optional Components:

1. pH Detector (optional, including flow cell and digital-to-analog converter);

2. Chromatography refrigerator;

3. ClearFirst (PC version) protein purification workstation;

4. Computer kit;


Product Parameters:

Solvent CompatibilityCommonly used aqueous buffer solutions for biomolecule purification
Flow Rate Range0-40 mL/min
Pressure Range0-0.2 MPa (2 bar, 30 psi)
Wavelength Range280 nm
Wavelength Accuracy±3 nm
Optical Path2 mm
Conductivity Range0-500 mS/cm
Friction CollectorSmall, fully automated fraction collector
Test Tube Racks
18 mm test tube rack (standard), 48 slots, suitable for 18*150 mm test tubes and 15 mL cell centrifuge tubes
1.5 mL test tube rack (optional), 130 slots, suitable for 1.5 mL centrifuge tubes
13 mm test tube rack (optional), 88 slots, suitable for 13*100 mm test tubes
15 mm test tube rack (optional), 80 slots, suitable for 15*150 mm test tubes and 5 mL cell centrifuge tubes
50 mL test tube rack (optional), 20 slots, suitable for 50 mL cell centrifuge tubes


AY-CF-500M Protein Purification System
AY-CF-500M Protein Purification System is a compact, integrated low-pressure protein chromatography system developed for small-scale protein purification in life science laboratories.
Designed to replace GE AKTA Start and Bio-Rad BioLogic LP, ClearFirst-500M integrates pumping, UV detection, conductivity monitoring, automated fraction collection and intelligent control into a single platform.
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         +86 18170839926                            +86 18079100967

     

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